PoplarV1, Main, Exploration, bibRecord, 001773

MicroRNA-mediated susceptible poplar gene expression regulation associated with the infection of virulent Melampsora larici-populina.

Identifieur interne : 001773 ( Main/Exploration ); précédent : 001772; suivant : 001774

MicroRNA-mediated susceptible poplar gene expression regulation associated with the infection of virulent Melampsora larici-populina.

Auteurs : Danlei Li [République populaire de Chine] ; Feng Wang [République populaire de Chine] ; Chao Wang [République populaire de Chine] ; Li Zou [République populaire de Chine] ; Zhiying Wang [République populaire de Chine] ; Qiaoli Chen [République populaire de Chine] ; Chunyang Niu [République populaire de Chine] ; Ruizhi Zhang [République populaire de Chine] ; Yaming Ling [République populaire de Chine] ; Bowen Wang [République populaire de Chine]

Source :

BMC genomics [ 1471-2164 ] ; 2016.

RBID : pubmed:26768277

Descripteurs français

KwdFr :
- Basidiomycota (génétique), Basidiomycota (pathogénicité), Feuilles de plante (génétique), Feuilles de plante (microbiologie), Immunité des plantes (génétique), Interactions hôte-pathogène (génétique), Maladies des plantes (génétique), Maladies des plantes (microbiologie), Populus (génétique), Populus (microbiologie), Régulation de l'expression des gènes végétaux (génétique), Résistance à la maladie (génétique), Transduction du signal (génétique), microARN (génétique).
MESH :
- génétique : Basidiomycota, Feuilles de plante, Immunité des plantes, Interactions hôte-pathogène, Maladies des plantes, Populus, Régulation de l'expression des gènes végétaux, Résistance à la maladie, Transduction du signal, microARN.
- microbiologie : Feuilles de plante, Maladies des plantes, Populus.
- pathogénicité : Basidiomycota.

English descriptors

KwdEn :
- Basidiomycota (genetics), Basidiomycota (pathogenicity), Disease Resistance (genetics), Gene Expression Regulation, Plant (genetics), Host-Pathogen Interactions (genetics), MicroRNAs (genetics), Plant Diseases (genetics), Plant Diseases (microbiology), Plant Immunity (genetics), Plant Leaves (genetics), Plant Leaves (microbiology), Populus (genetics), Populus (microbiology), Signal Transduction (genetics).
MESH :
- chemical , genetics : MicroRNAs.
- genetics : Basidiomycota, Disease Resistance, Gene Expression Regulation, Plant, Host-Pathogen Interactions, Plant Diseases, Plant Immunity, Plant Leaves, Populus, Signal Transduction.
- microbiology : Plant Diseases, Plant Leaves, Populus.
- pathogenicity : Basidiomycota.

Abstract

BACKGROUND

Rust caused by Melampsora larici-populina is one of the most damaging diseases of poplars. Rust is considered to be a model pathogen for genetic studies because both pathogen and host genomes are available. The poplar 'Robusta', whose general rust resistance is defeated by virulent rust E4, provides suitable host material for studies of the gene regulation involved in rust resistance/susceptibility. In this study, we investigated the microRNA-mediated susceptible poplar gene expression regulation associated with the infection of virulent rust. We were particularly interested in delineating the host-pathogen interactions with a specific focus on microRNAs (miRNAs).

RESULTS

To study the susceptibility of poplar to M. larici-populina, small RNA (sRNA) libraries, a degradome cDNA library and digital gene expression libraries were constructed for rust-inoculated and rust-free susceptible poplar 'Robusta' leaves through high-throughput sequencing. Altogether, 12,722 regulating interactions were identified. The results delineated the framework of post-transcriptional regulation of gene expression in the susceptible poplar, which was infected by the virulent rust. The results indicated that pathogen-associated molecular patterns (PAMPs) and PAMP-triggered immunity were induced by the infection of virulent rust E4 and that miRNAs still functioned at this stage. After this stage, miRNA-regulated R genes, such as TIR-NBS-LRR and CC-NBS-LRR, were not fully functional. Additionally, the rust-responsive miRNAs did not regulate the signaling component genes related to the salicylic acid pathway or the hypersensitive response.

CONCLUSIONS

We found that the defense-related post-transcriptional regulation of the susceptible poplar 'Robusta' functions normally only at the stage of PAMPs and PAMP-triggered immunity (PTI). More importantly, the miRNA-mediated post-transcriptional regulation of defense signal pathway genes were inactivated by the infection of virulent rust at the stage of effector-triggered susceptibility and during the following stages of salicylic acid and hypersensitive responses. This inactivation was the major characteristic of 'Robusta' susceptibility.

DOI: 10.1186/s12864-015-2286-6
PubMed: 26768277
PubMed Central: PMC4714501

Affiliations:

République populaire de Chine

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 001963
to stream Main, to step Curation: 001963

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">MicroRNA-mediated susceptible poplar gene expression regulation associated with the infection of virulent Melampsora larici-populina.</title>
<author><name sortKey="Li, Danlei" sort="Li, Danlei" uniqKey="Li D" first="Danlei" last="Li">Danlei Li</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Northeast Forestry University, Harbin, 150040, China. danleili@126.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin, 150040</wicri:regionArea>
<wicri:noRegion>150040</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wang, Feng" sort="Wang, Feng" uniqKey="Wang F" first="Feng" last="Wang">Feng Wang</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Northeast Forestry University, Harbin, 150040, China. kingsummit@126.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin, 150040</wicri:regionArea>
<wicri:noRegion>150040</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wang, Chao" sort="Wang, Chao" uniqKey="Wang C" first="Chao" last="Wang">Chao Wang</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Northeast Forestry University, Harbin, 150040, China. plantdisease@126.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin, 150040</wicri:regionArea>
<wicri:noRegion>150040</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zou, Li" sort="Zou, Li" uniqKey="Zou L" first="Li" last="Zou">Li Zou</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Northeast Forestry University, Harbin, 150040, China. rustfungi@163.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin, 150040</wicri:regionArea>
<wicri:noRegion>150040</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wang, Zhiying" sort="Wang, Zhiying" uniqKey="Wang Z" first="Zhiying" last="Wang">Zhiying Wang</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Northeast Forestry University, Harbin, 150040, China. nematodefungi@126.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin, 150040</wicri:regionArea>
<wicri:noRegion>150040</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chen, Qiaoli" sort="Chen, Qiaoli" uniqKey="Chen Q" first="Qiaoli" last="Chen">Qiaoli Chen</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Northeast Forestry University, Harbin, 150040, China. melampsora@126.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin, 150040</wicri:regionArea>
<wicri:noRegion>150040</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Niu, Chunyang" sort="Niu, Chunyang" uniqKey="Niu C" first="Chunyang" last="Niu">Chunyang Niu</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Northeast Forestry University, Harbin, 150040, China. virulencerust@126.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin, 150040</wicri:regionArea>
<wicri:noRegion>150040</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhang, Ruizhi" sort="Zhang, Ruizhi" uniqKey="Zhang R" first="Ruizhi" last="Zhang">Ruizhi Zhang</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Northeast Forestry University, Harbin, 150040, China. forestrydisease@163.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin, 150040</wicri:regionArea>
<wicri:noRegion>150040</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ling, Yaming" sort="Ling, Yaming" uniqKey="Ling Y" first="Yaming" last="Ling">Yaming Ling</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Northeast Forestry University, Harbin, 150040, China. kingrust@sina.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin, 150040</wicri:regionArea>
<wicri:noRegion>150040</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wang, Bowen" sort="Wang, Bowen" uniqKey="Wang B" first="Bowen" last="Wang">Bowen Wang</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Northeast Forestry University, Harbin, 150040, China. nematode2015@sina.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin, 150040</wicri:regionArea>
<wicri:noRegion>150040</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2016">2016</date>
<idno type="RBID">pubmed:26768277</idno>
<idno type="pmid">26768277</idno>
<idno type="doi">10.1186/s12864-015-2286-6</idno>
<idno type="pmc">PMC4714501</idno>
<idno type="wicri:Area/Main/Corpus">001963</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001963</idno>
<idno type="wicri:Area/Main/Curation">001963</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001963</idno>
<idno type="wicri:Area/Main/Exploration">001963</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">MicroRNA-mediated susceptible poplar gene expression regulation associated with the infection of virulent Melampsora larici-populina.</title>
<author><name sortKey="Li, Danlei" sort="Li, Danlei" uniqKey="Li D" first="Danlei" last="Li">Danlei Li</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Northeast Forestry University, Harbin, 150040, China. danleili@126.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin, 150040</wicri:regionArea>
<wicri:noRegion>150040</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wang, Feng" sort="Wang, Feng" uniqKey="Wang F" first="Feng" last="Wang">Feng Wang</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Northeast Forestry University, Harbin, 150040, China. kingsummit@126.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin, 150040</wicri:regionArea>
<wicri:noRegion>150040</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wang, Chao" sort="Wang, Chao" uniqKey="Wang C" first="Chao" last="Wang">Chao Wang</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Northeast Forestry University, Harbin, 150040, China. plantdisease@126.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin, 150040</wicri:regionArea>
<wicri:noRegion>150040</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zou, Li" sort="Zou, Li" uniqKey="Zou L" first="Li" last="Zou">Li Zou</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Northeast Forestry University, Harbin, 150040, China. rustfungi@163.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin, 150040</wicri:regionArea>
<wicri:noRegion>150040</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wang, Zhiying" sort="Wang, Zhiying" uniqKey="Wang Z" first="Zhiying" last="Wang">Zhiying Wang</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Northeast Forestry University, Harbin, 150040, China. nematodefungi@126.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin, 150040</wicri:regionArea>
<wicri:noRegion>150040</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chen, Qiaoli" sort="Chen, Qiaoli" uniqKey="Chen Q" first="Qiaoli" last="Chen">Qiaoli Chen</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Northeast Forestry University, Harbin, 150040, China. melampsora@126.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin, 150040</wicri:regionArea>
<wicri:noRegion>150040</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Niu, Chunyang" sort="Niu, Chunyang" uniqKey="Niu C" first="Chunyang" last="Niu">Chunyang Niu</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Northeast Forestry University, Harbin, 150040, China. virulencerust@126.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin, 150040</wicri:regionArea>
<wicri:noRegion>150040</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhang, Ruizhi" sort="Zhang, Ruizhi" uniqKey="Zhang R" first="Ruizhi" last="Zhang">Ruizhi Zhang</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Northeast Forestry University, Harbin, 150040, China. forestrydisease@163.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin, 150040</wicri:regionArea>
<wicri:noRegion>150040</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ling, Yaming" sort="Ling, Yaming" uniqKey="Ling Y" first="Yaming" last="Ling">Yaming Ling</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Northeast Forestry University, Harbin, 150040, China. kingrust@sina.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin, 150040</wicri:regionArea>
<wicri:noRegion>150040</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wang, Bowen" sort="Wang, Bowen" uniqKey="Wang B" first="Bowen" last="Wang">Bowen Wang</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Northeast Forestry University, Harbin, 150040, China. nematode2015@sina.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Forestry, Northeast Forestry University, Harbin, 150040</wicri:regionArea>
<wicri:noRegion>150040</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">BMC genomics</title>
<idno type="eISSN">1471-2164</idno>
<imprint><date when="2016" type="published">2016</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Basidiomycota (genetics)</term>
<term>Basidiomycota (pathogenicity)</term>
<term>Disease Resistance (genetics)</term>
<term>Gene Expression Regulation, Plant (genetics)</term>
<term>Host-Pathogen Interactions (genetics)</term>
<term>MicroRNAs (genetics)</term>
<term>Plant Diseases (genetics)</term>
<term>Plant Diseases (microbiology)</term>
<term>Plant Immunity (genetics)</term>
<term>Plant Leaves (genetics)</term>
<term>Plant Leaves (microbiology)</term>
<term>Populus (genetics)</term>
<term>Populus (microbiology)</term>
<term>Signal Transduction (genetics)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Basidiomycota (génétique)</term>
<term>Basidiomycota (pathogénicité)</term>
<term>Feuilles de plante (génétique)</term>
<term>Feuilles de plante (microbiologie)</term>
<term>Immunité des plantes (génétique)</term>
<term>Interactions hôte-pathogène (génétique)</term>
<term>Maladies des plantes (génétique)</term>
<term>Maladies des plantes (microbiologie)</term>
<term>Populus (génétique)</term>
<term>Populus (microbiologie)</term>
<term>Régulation de l'expression des gènes végétaux (génétique)</term>
<term>Résistance à la maladie (génétique)</term>
<term>Transduction du signal (génétique)</term>
<term>microARN (génétique)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>MicroRNAs</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Basidiomycota</term>
<term>Disease Resistance</term>
<term>Gene Expression Regulation, Plant</term>
<term>Host-Pathogen Interactions</term>
<term>Plant Diseases</term>
<term>Plant Immunity</term>
<term>Plant Leaves</term>
<term>Populus</term>
<term>Signal Transduction</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Basidiomycota</term>
<term>Feuilles de plante</term>
<term>Immunité des plantes</term>
<term>Interactions hôte-pathogène</term>
<term>Maladies des plantes</term>
<term>Populus</term>
<term>Régulation de l'expression des gènes végétaux</term>
<term>Résistance à la maladie</term>
<term>Transduction du signal</term>
<term>microARN</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Feuilles de plante</term>
<term>Maladies des plantes</term>
<term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term>
<term>Plant Leaves</term>
<term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Basidiomycota</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Basidiomycota</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b>
</p>
<p>Rust caused by Melampsora larici-populina is one of the most damaging diseases of poplars. Rust is considered to be a model pathogen for genetic studies because both pathogen and host genomes are available. The poplar 'Robusta', whose general rust resistance is defeated by virulent rust E4, provides suitable host material for studies of the gene regulation involved in rust resistance/susceptibility. In this study, we investigated the microRNA-mediated susceptible poplar gene expression regulation associated with the infection of virulent rust. We were particularly interested in delineating the host-pathogen interactions with a specific focus on microRNAs (miRNAs).</p>
</div>
<div type="abstract" xml:lang="en"><p><b>RESULTS</b>
</p>
<p>To study the susceptibility of poplar to M. larici-populina, small RNA (sRNA) libraries, a degradome cDNA library and digital gene expression libraries were constructed for rust-inoculated and rust-free susceptible poplar 'Robusta' leaves through high-throughput sequencing. Altogether, 12,722 regulating interactions were identified. The results delineated the framework of post-transcriptional regulation of gene expression in the susceptible poplar, which was infected by the virulent rust. The results indicated that pathogen-associated molecular patterns (PAMPs) and PAMP-triggered immunity were induced by the infection of virulent rust E4 and that miRNAs still functioned at this stage. After this stage, miRNA-regulated R genes, such as TIR-NBS-LRR and CC-NBS-LRR, were not fully functional. Additionally, the rust-responsive miRNAs did not regulate the signaling component genes related to the salicylic acid pathway or the hypersensitive response.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b>
</p>
<p>We found that the defense-related post-transcriptional regulation of the susceptible poplar 'Robusta' functions normally only at the stage of PAMPs and PAMP-triggered immunity (PTI). More importantly, the miRNA-mediated post-transcriptional regulation of defense signal pathway genes were inactivated by the infection of virulent rust at the stage of effector-triggered susceptibility and during the following stages of salicylic acid and hypersensitive responses. This inactivation was the major characteristic of 'Robusta' susceptibility.</p>
</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26768277</PMID>
<DateCompleted><Year>2016</Year>
<Month>09</Month>
<Day>28</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1471-2164</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>17</Volume>
<PubDate><Year>2016</Year>
<Month>Jan</Month>
<Day>15</Day>
</PubDate>
</JournalIssue>
<Title>BMC genomics</Title>
<ISOAbbreviation>BMC Genomics</ISOAbbreviation>
</Journal>
<ArticleTitle>MicroRNA-mediated susceptible poplar gene expression regulation associated with the infection of virulent Melampsora larici-populina.</ArticleTitle>
<Pagination><MedlinePgn>59</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1186/s12864-015-2286-6</ELocationID>
<Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Rust caused by Melampsora larici-populina is one of the most damaging diseases of poplars. Rust is considered to be a model pathogen for genetic studies because both pathogen and host genomes are available. The poplar 'Robusta', whose general rust resistance is defeated by virulent rust E4, provides suitable host material for studies of the gene regulation involved in rust resistance/susceptibility. In this study, we investigated the microRNA-mediated susceptible poplar gene expression regulation associated with the infection of virulent rust. We were particularly interested in delineating the host-pathogen interactions with a specific focus on microRNAs (miRNAs).</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">To study the susceptibility of poplar to M. larici-populina, small RNA (sRNA) libraries, a degradome cDNA library and digital gene expression libraries were constructed for rust-inoculated and rust-free susceptible poplar 'Robusta' leaves through high-throughput sequencing. Altogether, 12,722 regulating interactions were identified. The results delineated the framework of post-transcriptional regulation of gene expression in the susceptible poplar, which was infected by the virulent rust. The results indicated that pathogen-associated molecular patterns (PAMPs) and PAMP-triggered immunity were induced by the infection of virulent rust E4 and that miRNAs still functioned at this stage. After this stage, miRNA-regulated R genes, such as TIR-NBS-LRR and CC-NBS-LRR, were not fully functional. Additionally, the rust-responsive miRNAs did not regulate the signaling component genes related to the salicylic acid pathway or the hypersensitive response.</AbstractText>
<AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">We found that the defense-related post-transcriptional regulation of the susceptible poplar 'Robusta' functions normally only at the stage of PAMPs and PAMP-triggered immunity (PTI). More importantly, the miRNA-mediated post-transcriptional regulation of defense signal pathway genes were inactivated by the infection of virulent rust at the stage of effector-triggered susceptibility and during the following stages of salicylic acid and hypersensitive responses. This inactivation was the major characteristic of 'Robusta' susceptibility.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName>
<ForeName>Danlei</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>College of Forestry, Northeast Forestry University, Harbin, 150040, China. danleili@126.com.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Feng</ForeName>
<Initials>F</Initials>
<AffiliationInfo><Affiliation>College of Forestry, Northeast Forestry University, Harbin, 150040, China. kingsummit@126.com.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Chao</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>College of Forestry, Northeast Forestry University, Harbin, 150040, China. plantdisease@126.com.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zou</LastName>
<ForeName>Li</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>College of Forestry, Northeast Forestry University, Harbin, 150040, China. rustfungi@163.com.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Zhiying</ForeName>
<Initials>Z</Initials>
<AffiliationInfo><Affiliation>College of Forestry, Northeast Forestry University, Harbin, 150040, China. nematodefungi@126.com.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chen</LastName>
<ForeName>Qiaoli</ForeName>
<Initials>Q</Initials>
<AffiliationInfo><Affiliation>College of Forestry, Northeast Forestry University, Harbin, 150040, China. melampsora@126.com.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Niu</LastName>
<ForeName>Chunyang</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>College of Forestry, Northeast Forestry University, Harbin, 150040, China. virulencerust@126.com.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhang</LastName>
<ForeName>Ruizhi</ForeName>
<Initials>R</Initials>
<AffiliationInfo><Affiliation>College of Forestry, Northeast Forestry University, Harbin, 150040, China. forestrydisease@163.com.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ling</LastName>
<ForeName>Yaming</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>College of Forestry, Northeast Forestry University, Harbin, 150040, China. kingrust@sina.com.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Bowen</ForeName>
<Initials>B</Initials>
<AffiliationInfo><Affiliation>College of Forestry, Northeast Forestry University, Harbin, 150040, China. nematode2015@sina.com.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2016</Year>
<Month>01</Month>
<Day>15</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>BMC Genomics</MedlineTA>
<NlmUniqueID>100965258</NlmUniqueID>
<ISSNLinking>1471-2164</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D035683">MicroRNAs</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D001487" MajorTopicYN="N">Basidiomycota</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D060467" MajorTopicYN="N">Disease Resistance</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D054884" MajorTopicYN="N">Host-Pathogen Interactions</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D035683" MajorTopicYN="N">MicroRNAs</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D057865" MajorTopicYN="N">Plant Immunity</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015398" MajorTopicYN="N">Signal Transduction</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year>
<Month>08</Month>
<Day>26</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2015</Year>
<Month>12</Month>
<Day>10</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2016</Year>
<Month>1</Month>
<Day>16</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2016</Year>
<Month>1</Month>
<Day>16</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2016</Year>
<Month>9</Month>
<Day>30</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>epublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">26768277</ArticleId>
<ArticleId IdType="doi">10.1186/s12864-015-2286-6</ArticleId>
<ArticleId IdType="pii">10.1186/s12864-015-2286-6</ArticleId>
<ArticleId IdType="pmc">PMC4714501</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Curr Opin Plant Biol. 2007 Aug;10(4):372-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17646123</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Biol. 2008 May 20;18(10):758-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18472421</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 1999 Aug;11(8):1393-404</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10449575</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2007 May;144(1):347-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17400708</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2010 Apr;22(4):1090-103</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20407023</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Plant Biol. 2001 Aug;4(4):301-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11418339</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2008 Jul;55(1):131-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18363789</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2010 Jun 1;62(6):960-76</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20230504</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Genomics. 2013;14:233</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23570526</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genome Biol. 2010;11(10):R106</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20979621</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Plant Biol. 2009 Aug;12(4):465-72</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19608454</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Med. 2012 Feb;18(2):267-73</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22286305</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Genomics. 2007;8:481</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18166134</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Bioinformatics. 2009 Jan 1;25(1):130-1</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19017659</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2006 Nov 16;444(7117):323-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17108957</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Phytopathol. 2005;43:205-27</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16078883</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Dev Biol. 2006 Jan 1;289(1):3-16</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16325172</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2001 Jun 14;411(6839):826-33</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11459065</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Genomics. 2013;14:9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23324572</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Plant Biol. 2006;57:19-53</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16669754</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2007;2(2):e219</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17299599</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Pathog. 2011;2011:716041</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22567338</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2013;8(12):e84390</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24391949</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genome Res. 2008 Oct;18(10):1602-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18653800</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2012;7(9):e44968</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23028709</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant Microbe Interact. 2011 Jul;24(7):808-18</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21644839</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Phytopathology. 1999 Mar;89(3):233-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18944764</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant Microbe Interact. 2010 Oct;23(10):1275-86</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20831407</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell. 2004 Jun 18;14(6):787-99</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15200956</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Immunol. 2011 Sep;12(9):861-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21785411</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Mol Biol. 2008 Apr;66(6):619-36</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18247136</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2009 May 8;324(5928):750-2</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19423816</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Planta. 2009 Mar;229(4):1009-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19148671</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>FEBS Lett. 2008 Jul 9;582(16):2445-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18558089</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2002 Sep;14(9):1983-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12215498</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2010;5(10):e13669</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21060679</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genes Dev. 2006 Dec 15;20(24):3407-25</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17182867</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Plant Biol. 2008 Aug;11(4):464-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18583181</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2012;7(8):e44408</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22952974</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2006 Apr 21;312(5772):436-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16627744</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2012 Mar;24(3):859-74</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22408077</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>République populaire de Chine</li>
</country>
</list>
<tree><country name="République populaire de Chine"><noRegion><name sortKey="Li, Danlei" sort="Li, Danlei" uniqKey="Li D" first="Danlei" last="Li">Danlei Li</name>
</noRegion>
<name sortKey="Chen, Qiaoli" sort="Chen, Qiaoli" uniqKey="Chen Q" first="Qiaoli" last="Chen">Qiaoli Chen</name>
<name sortKey="Ling, Yaming" sort="Ling, Yaming" uniqKey="Ling Y" first="Yaming" last="Ling">Yaming Ling</name>
<name sortKey="Niu, Chunyang" sort="Niu, Chunyang" uniqKey="Niu C" first="Chunyang" last="Niu">Chunyang Niu</name>
<name sortKey="Wang, Bowen" sort="Wang, Bowen" uniqKey="Wang B" first="Bowen" last="Wang">Bowen Wang</name>
<name sortKey="Wang, Chao" sort="Wang, Chao" uniqKey="Wang C" first="Chao" last="Wang">Chao Wang</name>
<name sortKey="Wang, Feng" sort="Wang, Feng" uniqKey="Wang F" first="Feng" last="Wang">Feng Wang</name>
<name sortKey="Wang, Zhiying" sort="Wang, Zhiying" uniqKey="Wang Z" first="Zhiying" last="Wang">Zhiying Wang</name>
<name sortKey="Zhang, Ruizhi" sort="Zhang, Ruizhi" uniqKey="Zhang R" first="Ruizhi" last="Zhang">Ruizhi Zhang</name>
<name sortKey="Zou, Li" sort="Zou, Li" uniqKey="Zou L" first="Li" last="Zou">Li Zou</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001773 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001773 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Bois
   |area=    PoplarV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:26768277
   |texte=   MicroRNA-mediated susceptible poplar gene expression regulation associated with the infection of virulent Melampsora larici-populina.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:26768277" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a PoplarV1

This area was generated with Dilib version V0.6.37.
Data generation: Wed Nov 18 12:07:19 2020. Site generation: Wed Nov 18 12:16:31 2020

	Serveur d'exploration sur le peuplier
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur le peuplier

MicroRNA-mediated susceptible poplar gene expression regulation associated with the infection of virulent Melampsora larici-populina.

MicroRNA-mediated susceptible poplar gene expression regulation associated with the infection of virulent Melampsora larici-populina.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki